![]() Products can be purchased ala carte or as a bundled system with ScanImage Premium or SI 5.įor technical information about ScanImage Premium and SI 5, we direct you to the Vidrio and ScanImage web sites. Sutter provides hardware that operates seamlessly with all versions of ScanImage. We are grateful to have had a mutual, continuing connection to ScanImage and the ScanImage community for over ten years. In addition to the MOM® in vivo two-photon scope, Sutter has many products that are used by two-photon microscopists using ScanImage including our stepper motor drive XYZ controllers, galvo and resonant scanner systems, PMTs and PMT power supplies. Two-photon microscope development at Sutter Instrument has been closely tied to ScanImage software for years. Ultimately, ScanImage support and development has become a commercial enterprise and Vidrio is the new face of ScanImage. However, as ScanImage became increasingly popular and as new versions were required to cover changes in operating systems and hardware drivers, it soon became obvious that a high level of maintenance could not be sustained based on the “freeware model”. ScanImage was designed to be a freely available open source program and its early maintenance was “easily” supported. It is hard to over-estimate the influence of this software in the field of two-photon scanning microscopy. 66, 486–501 (2010).ScanImage is imaging software for scanning microscopes developed by Karel Svoboda and coworkers (Pologruto et al, 2003) and judiciously maintained by members of the Svoboda lab, first at CSHL and then at Janelia Farms, HHMI. Refinement of macromolecular structures by the maximum-likelihood method. Overview of the CCP4 suite and current developments. iMOSFLM: a new graphical interface for diffraction-image processing with MOSFLM. G., Kontogiannis, L., Johnson, O., Powell, H. Reevaluation of absolute luminescence quantum yields of standard solutions using a spectrometer with an integrating sphere and a back-thinned CCD detector. NMR chemical shifts of common laboratory solvents as trace impurities. A general method to optimize and functionalize red-shifted rhodamine dyes. Fluorescence ratio imaging of cyclic AMP in single cells. Optically detected structural change in the N-terminal region of the voltage-sensor domain. Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe. A neuron-based screening platform for optimizing genetically-encoded calcium indicators. A survey of Hammett substituent constants and resonance and field parameters. Synthesis of rhodamines from fluoresceins using Pd-catalyzed C–N cross-coupling. Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. Ultrasensitive fluorescent proteins for imaging neuronal activity. Circular permutation and receptor insertion within green fluorescent proteins. Teaching old dyes new tricks: biological probes built from fluoresceins and rhodamines. A general method to fine-tune fluorophores for live-cell and in vivo imaging. A general method to improve fluorophores for live-cell and single-molecule microscopy. Bright and photostable chemigenetic indicators for extended in vivo voltage imaging. HaloTag technology: a versatile platform for biomedical applications. Beyond fluorescent proteins: hybrid and bioluminescent indicators for imaging neural activities. Chemical tags: applications in live cell fluorescence imaging. Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands. HaloTag: a novel protein labeling technology for cell imaging and protein analysis. Covalently tethered rhodamine voltage reporters for high speed functional imaging in brain tissue. Spying on neuronal membrane potential with genetically targetable voltage indicators. Isomeric tuning yields bright and targetable red Ca 2+ indicators. Fluorogenic targeting of voltage-sensitive dyes to neurons. Liu, P., Grenier, V., Hong, W., Muller, V. Single-trial imaging of spikes and synaptic potentials in single neurons in brain slices with genetically encoded hybrid voltage sensor. Genetic targeting of a voltage-sensitive dye by enzymatic activation of phosphonooxymethyl-ammonium derivative. A genetically encoded near-infrared fluorescent calcium ion indicator. Sensitive red protein calcium indicators for imaging neural activity. High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor. A high signal-to-noise Ca 2+ probe composed of a single green fluorescent protein. High-performance calcium sensors for imaging activity in neuronal populations and microcompartments. Genetically encoded indicators of neuronal activity.
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